Table 1.
Antioxidant activity (as % and IC50) of the four promising algal fractions from the crude extract from {(0.316 mg/L (Cu) of C.vulgaris compared to BHT and vitamin C as standards against ABTS (%) radical method
| Fraction | Hexane (100%) | Hexane:Chloroform (6:4) | Hexane:Chloroform (5:5) |
Hexane:Chloroform (4:6) |
BHT | Vit.C |
|---|---|---|---|---|---|---|
| Conc. (μg/ml) | ||||||
| 500 | 94.013±1.00a | 96.220±1.070a | 97.203±1.06a | 94.253±1.092a | 94.3±3.0a | 97.1±3.6a |
| 250 | 93.110±1.01a | 95.237±1.081a | 96.220±1.07a | 89.013±1.000b | 90.8±2.9b | 93.2±2.5b |
| 125 | 80.000±1.00b | 85.157±1.036b | 92.283±1.11b | 85.110±1.018c | 85.4±2.0c | 90.2±2.1c |
| 62.50 | 54.947±0.88c | 68.170±0.662c | 86.203±0.621c | 71.073±0.793d | 70.7±1.0d | 87.9±1.9d |
| 31.25 | 51.077±0.88d | 56.000±1.000d | 80.410±0.524d | 66.190±0.734e | 57.5±0.9e | 76.9±1.9e |
| 15.62 | 39.333±0.57e | 40.610±0.385e | 53.000±1.00e | 44.600±0.529f | 51.8±0.9f | 53.0±1.0f |
| IC50 | 19.85 | 19.52 | 14.2 | 17.75 | 15.1 | 14.7 |
Different small letters on the column for each fraction indicate significant difference (p< 0.05). Error bars represent ±SD of three replicates