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. 2020 Dec 14;13(2):e10852. doi: 10.15252/emmm.201910852

Figure 4. MRP1 inhibition increases GS‐MQ content, forming a reservoir of MQ for interaction with other targets.

Figure 4

  1. APR‐246 content in OVCAR‐3 cells (TP53 R248Q) 24 h after treatment with APR‐246 +/− MK‐571 (n = 3).
  2. Glutathione‐conjugated MQ (GS‐MQ) in OVCAR‐3 cells after 24‐h treatment with APR‐246 +/− MK‐571 (n = 2).
  3. Amount of GS‐MQ at indicated timepoints after incubation of GS‐MQ +/− NAC (n = 2). See Fig EV4 for chemical reaction.
  4. Amount of GS‐MQ by LC‐MS (green line, left axis) and NAC‐MQ (purple line, right axis) over time after incubation of GS‐MQ with NAC (n = 2). Values on y‐axes are not comparable because GS‐MQ and NAC‐MQ have different response signals on MS.

Data information: Indicated values are assessed by LC‐MS. Data are represented as mean ± SEM. See also Appendix Fig S4.