Figure EV3. Restoration of variant CSF‐1R levels does not restore function.

• A–C
  qPCR of wild type (blue) or Csf1r ^+/− (red) endothelial cells treated with control, Csf1r ^+/+ MCM or Csf1r ^+/− MCM. Statistical analyses of inter‐genotype changes. (*P < 0.05, **P < 0.005, ***P < 0.0008, ****P < 0.0001. Scatter plots represent technical replicates of n = 2 independent primary cell isolations and microglia conditionings, two‐way ANOVA with multiple comparisons and Sidak’s post‐test, error bars indicate SEM).Western blot for phosphorylated and total ERK in HEK293 cells transfected with (A) native CSF‐1R, (B) ΔA781_N783 CSF‐1R or (C) both native and ΔA781_N783 CSF‐1R. Cells were treated with MG132 for 1 h, followed by treatment with CSF1 for 10 min. Horizontal line indicates untreated cells, with increasing concentrations 10, 50 and 100 ng/ml CSF1.