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. 2020 Dec 22;13(2):e12889. doi: 10.15252/emmm.202012889

Figure 4. CSF‐1R expression in endothelial cells and macrophages affects response to amyloid injection.

Figure 4

  1. Immunohistochemistry of Csf‐1rflx / wt;Cx3cr1‐Cre − (top) and Csf‐1rflx / wt;Cx3cr1‐Cre + (bottom) mice unilaterally injected with Aβ1‐42 in the hippocampus and stained for IB4 (green), F4/80 (white) and Aβ (red). Scale bars indicate 50 μm.
  2. Quantification of F4/80 and IB4 immunopositivity following intrahippocampal injection of Aβ1‐42. Quantification of immunopositivity in both injected and uninjected hippocampi of Csf‐1rflx / wt;Cx3cr1‐Cre − and Csf‐1rflx / wt;Cx3cr1‐Cre + mice. (Two‐way ANOVA with Sidak’s test for multiple comparisons. Asterisks (*) indicate comparison to immunopositivity values of the uninjected hippocampus, obliques (#) indicate comparison between Csf‐1rflx / wt;Cx3cr1‐Cre − and Csf‐1rflx / wt;Cx3cr1‐Cre + mice, ## or **P < 0.003, #### or ****P < 0.0001, n = 5 mice per group, error bars indicate SEM.)
  3. Immunohistochemistry of Csf‐1rflx / wt; Tie2‐Cre − (top) and Csf‐1rflx / wt;Tie2‐Cre + mice (bottom) mice unilaterally injected with Aβ1‐42 in the hippocampus and stained for IB4 (green), F4/80 (white) and Aβ (red).
  4. Quantification of F4/80 and IB4 immunopositivity following intrahippocampal injection of Aβ1‐42. Quantification of immunopositivity in both injected and uninjected hippocampi of Csf‐1rflx / wt;Tie2‐Cre − and Csf‐1rflx / wt;Tie2‐Cre + mice. (n = 5 mice per group. Two‐way ANOVA with Sidak’s test for multiple comparisons. Asterisks (*) indicate comparison to immunopositivity values of the uninjected hippocampus, *P < 0.01, ***P < 0.0005, error bars indicate SEM).