Figure EV3. PSMA RNA expression and membrane staining on lung SCC cryosections.

• A
  RNA in situ hybridization was performed using the RNAscope® kit and probes against PSMA, DapB (negative control), and UBC (positive control) as described in the Materials and Methods section. Scale 10 µm.
• B
  Lung SSC tumor sections, (upper panel with PSMA‐positive tumor lower panel with PSMA‐negative tumor), were stained with 10B3 and a Cy3‐labeled secondary antibody followed by a FITC‐labeled antibody to the human EpCAM molecule. Scale 10 µm.