Figure EV4. Consequences of Fc silencing and chelation of CC‐1.

• A
  Binding of CC‐1 (FcKO) and a variant containing a wild type Fc part (FcWT) to the indicated his‐tagged FcR was determined by ELISA. All experiments were performed at a neutral pH except for FcRn, where binding was additionally evaluated at a pH of 6. Means of duplicate measurements are shown.
• B, C
  BsAbs in the Fabsc and IgGsc format chelated or not to p‐NCS‐Bn‐NODAGA were incubated with (B) 22Rv1^high (PSMA⁺) cells or (C) Jurkat (CD3⁺) cells followed by binding analysis by flow cytometry.
• D
  LNCaP cells were incubated with PBMC and the indicated concentrations of chelated or native bsAbs, and T‐cell activation was assessed using a ³H thymidine uptake assay.