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. 2020 Dec 22;13(2):e13259. doi: 10.15252/emmm.202013259

Figure 6. AAV.Syne4 promotes long‐term survival of OHC in Syne4 −/− mice.

Figure 6

  1. Whole‐mount immunofluorescence at 12w of the organ of Corti of a Syne4 −/− mouse injected with AAV.Syne4 mouse injected with AAV.Syne4.
  2. Whole‐mount immunofluorescence at 12w of the 12 kHz region of WT, Syne4 −/− mouse, and Syne4 −/− mouse injected with AAV.Syne4.
  3. IHC and OHC counts, n = 3 for WT, n = 4 for Mut, and n = 6 for Mut + AAV.Syne4.
  4. Correlation between HC count and ABR threshold at 12kHz.
  5. 3D surface projection of an OHC at 12w from the 12kHz region of WT and Syne4 −/− mice injected with AAV.Syne4. Open arrows denote basal end, and white arrows denote cuticular plate.
  6. Image analysis of nucleus position of OHC.
  7. Quantification of nuclear distance from the cuticular plate. A total of 30 OHC from 3 WT mice and 38 OHC from 4 Syne4 −/− mice injected with AAV.Syne4 were measured.

Data information: Scale bars = 100 μm for (A), 10 μm for (B), and 5 μm for (E). Statistical tests were Pearson correlation with two‐tailed P values for (D) and unpaired Student’s t‐test for (G). Plots show mean ± SD.

Source data are available online for this figure.