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. 2020 Dec 17;13(2):e12640. doi: 10.15252/emmm.202012640

Figure EV3. SP1 expression is lost following mithramycin treatment but does not affect cell proliferation.

Figure EV3

  1. SP1 mRNA expression is reduced following 100 nM mithramycin treatment as measured by qPCR fold change relative to GAPDH (2ddCT) (8 h, P = 0.0001; 18 h, P = 0.0001). Data represent mean with standard deviation derived from three independent experiments. P‐values were determined by one‐way ANOVA using Dunnett test for multiple comparisons.
  2. SP1 expression is dependent on SWI/SNF. siRNA silencing of SMARCC1 and SMARCA4 subunits is associated with a similar loss of SP1 expression as direct silencing of SP1 as measured by qPCR relative to GAPDH (2ddCT). ****P = 0.0001. Data represent mean with standard deviation derived from three independent experiments. P‐values were determined by one‐way ANOVA using Dunnett test for multiple comparisons.
  3. Knockdown of SP1 (black) does not affect BT12 rhabdoid tumor cell proliferation compared with a siNeg (gray, solid line) negative control as measured by live cell imaging. siDeath (gray, dotted line) is a positive control for knockdown efficiency. Data represent mean with standard deviation derived from three independent experiments.