Figure 7. FAM210A interacts with EF‐Tu and regulates mitochondrial‐encoded protein expression.

• A
  Antibody‐based affinity purification of endogenous FAM210A from purified murine cardiac mitochondria coupled with mass spectrometry analysis.
• B
  Top list of FAM210A‐interacting proteins in IP‐MS (immunoprecipitation‐mass spectrometry) analysis. PSM: peptide spectrum match.
• C
  Confocal imaging of co‐localization of FAM210A‐3xFLAG and endogenous EF‐Tu by immunostaining. Scale bar: 40 μm.
• D
  Left panel: IP‐IB validates FAM210A‐interacting proteins using pre‐immune IgG and FAM210A antibodies. Right panel: IP‐IB validates FAM210A‐interacting proteins in HEK293T cell lysates subjected to IP using pre‐immune IgG and anti‐FLAG antibodies. A representative image is shown in three biological replicates.
• E
  Mapping of interacting domains in FAM210A for binding EF‐Tu.
• F, G
  Protein expression of mitochondrial ETC component genes was measured in AC16 cells after following treatments in comparison with control cells: (F) siRNA knockdown of FAM210A. (G) FAM210A overexpression. Quantitative data were shown in Appendix Fig S4A and B.

Source data are available online for this figure.