Table 1.
Cardiac function of WT and miR‐574−/− mice under TAC surgery
| Parameter | WT, Sham (n = 8) | WT, TAC (n = 8) | miR‐574−/−, Sham (n = 8) | miR‐574−/−, TAC (n = 8) | ||||
|---|---|---|---|---|---|---|---|---|
| BL | 4 week | BL | 4 week | BL | 4 week | BL | 4 week | |
| HR (BPM) | 563.04 ± 7.02 | 582.30 ± 8.80 | 543.03 ± 7.80 | 558.54 ± 4.41 | 552.07 ± 8.01 | 564.70 ± 9.32 | 544.79 ± 9.78 | 547.87 ± 8.47 |
| LVID,s (mm) | 1.64 ± 0.161 | 2.04 ± 0.146 | 2.01 ± 0.065 | 3.02 ± 0.309* | 1.91 ± 0.107 | 1.86 ± 0.138 | 1.91 ± 0.088 | 3.75 ± 0.212 †† |
| LVID,d (mm) | 3.07 ± 0.278 | 3.27 ± 0.092 | 3.15 ± 0.096 | 3.85 ± 0.298 | 3.31 ± 0.108 | 3.18 ± 0.096 | 3.25 ± 0.084 | 4.91 ± 0.162 † |
| LVV,s (μL) | 7.91 ± 0.41 | 8.98 ± 0.45 | 11.27 ± 0.79 | 25.48 ± 5.04*** | 8.17 ± 0.66 | 8.78 ± 1.30 | 14.13 ± 1.72 | 47.12 ± 3.04 ††† |
| LVV,d (μL) | 40.65 ± 2.04 | 41.58 ± 1.76 | 41.76 ± 1.97 | 55.62 ± 6.10** | 39.58 ± 2.90 | 41.02 ± 2.50 | 48.07 ± 2.90 | 77.47 ± 4.25 ††† |
| SV (μL) | 29.60 ± 2.934 | 32.64 ± 2.011 | 33.12 ± 2.478 | 28.47 ± 2.995 | 33.28 ± 2.260 | 32.17 ± 1.306 | 31.05 ± 1.511 | 26.73 ± 2.683 |
| EF (%) | 79.98 ± 0.97 | 79.44 ± 0.47 | 79.00 ± 0.67 | 57.50 ± 2.20*** | 78.50 ± 0.78 | 78.37 ± 0.49 | 78.90 ± 0.82 | 46.25 ± 1.68 †† |
| FS (%) | 45.46 ± 0.26 | 46.93 ± 0.36 | 45.00 ± 0.58 | 34.00 ± 0.80*** | 44.70 ± 0.33 | 46.75 ± 0.37 | 45.44 ± 0.35 | 26.50 ± 1.40 ††† |
| CO (mL/min) | 16.49 ± 1.633 | 18.77 ± 1.312 | 17.16 ± 1.003 | 16.00 ± 1.532 | 18.62 ± 1.225 | 17.47 ± 0.866 | 17.79 ± 1.013 | 15.61 ± 1.626 |
| LVM (mg) | 55.53 ± 5.318 | 58.2 ± 3.40 | 60.04 ± 3.403 | 120.82 ± 8.585*** | 61.42 ± 2.866 | 62.7 ± 4.120 | 60.26 ± 3.868 | 155.99 ± 11.734 †† |
| LVAWD,s (mm) | 1.05 ± 0.02 | 1.09 ± 0.03 | 0.90 ± 0.05 | 1.12 ± 0.06 | 1.06 ± 0.03 | 0.93 ± 0.06 | 0.83 ± 0.04 | 0.98 ± 0.06 |
| LVAWD,d (mm) | 0.67 ± 0.02 | 0.71 ± 0.02 | 0.77 ± 0.04 | 1.00 ± 0.05* | 0.74 ± 0.03 | 0.76 ± 0.04 | 0.68 ± 0.02 | 0.83 ± 0.05 † |
| LVPWD,s (mm) | 0.87 ± 0.02 | 0.89 ± 0.02 | 0.88 ± 0.04 | 1.07 ± 0.04 | 0.86 ± 0.02 | 0.90 ± 0.04 | 0.84 ± 0.04 | 0.91 ± 0.06 |
| LVPWD,d (mm) | 0.55 ± 0.01 | 0.55 ± 0.01 | 0.70 ± 0.03 | 0.94 ± 0.03** | 0.54 ± 0.01 | 0.60 ± 0.05 | 0.68 ± 0.02 | 0.83 ± 0.05 |
| BW (g) | 25.60 ± 0.46 | 26.90 ± 0.52 | 26.02 ± 1.01 | 25.80 ± 0.74 | 25.90 ± 0.52 | 27.30 ± 0.70 | 26.06 ± 0.31 | 25.87 ± 0.50 |
BW, Body Weight (g); CO, Cardiac Output (ml/min); EF, Ejection Fraction (%); FS, Fractional Shortening (%); HR, Heart Rate (BPM, beats per minute); LVAWD,d, Left Ventricular Anterior Wall Diameter, diastole (mm); LVAWD,s, Left Ventricular Anterior Wall Diameter, systole (mm); LVID,d, Left Ventricular Internal Diameter, diastole (mm); LVID,s, Left Ventricular Internal Diameter, systole (mm); LVM, LV Mass (mg); LVPWD,d, Left Ventricular Posterior Wall Diameter, diastole (mm); LVPWD,s, Left Ventricular Posterior Wall Diameter, systole (mm); LVV,d, Left Ventricular Volume, diastole (μl); LVV,s, Left Ventricular Volume, systole (μl); SV, Stroke Volume (μl).
Values are expressed as mean ± SEM.
P < 0.05, **P < 0.01, ***P < 0.001 for WT, TAC vs. WT, Sham.
P < 0.05, †† P < 0.01, ††† P < 0.001 for miR‐574−/−, TAC vs. WT, TAC.