Table 3.
Primer sequences of RT-qPCR used for the analyses of inflammatory and anti-inflammatory gene expressions of LPS-stimulated THP-1 macrophage cells
| Specific gene | Primer | |
|---|---|---|
| Cytokines gene | IL-1β |
F-GTGGCAATGAGGATGACTTGTTC R-TAGTGGTGGTCGGAGATTCGTA |
| IL-6 |
F-AGCCACTCACCTCTTCAGAAC R-GCCTCTTTGCTGCTTTCACAC |
|
| IL-8 |
F-CTGATTTCTGCAGCTCTGTG R-GGGTGGAAAGGTTTGGAGTATG |
|
| IL-10 |
F-GTGATGCCCCAAGCTGAGA R-CACGGCCTTGCTCTTGTTTT |
|
| TNF-α |
F-CTGCTGCACT TTGGAGTGAT R-AGATGATCTG ACTGCCTGGG |
|
| Housekeeping gene | ACTB (β-actin) |
F-ATTGCCGACAGGATGCAGAA R-GCTGATCCACATCTGCTGGAA |
|
GAPDH (Glyceraldehyde-3-phosphatedehydrogenase) |
F-CAACAGCGACACCCACTCCT R-CACCCTGTTGCTGTAGCCAAA |