Fig. 3.

Lentiviral transduction does not result in stable light-activated gene expression. a DG44 and b CHO-K1 cells serially transduced with lentiviral vectors encoding the LACE system were incubated in the dark or with light activation for 24 h. Fluorescence intensity of cells was measured by flow cytometry. c Cells in the top 0.1% of fluorescence from (b) were single-cell sorted and expanded. The surviving clonal populations were incubated in the dark or with light activation for 24 h. Fluorescence intensity of cells was measured by flow cytometry. Transient transfection data from Fig. 2a is included as a reference for robust light activation