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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Chemosphere. 2020 Jan 3;247:125825. doi: 10.1016/j.chemosphere.2020.125825

Fig. 5. Anacardic Acid (AA) and MG149 inhibited fluoride-induced cytochrome-c release in LS8 cells.

Fig. 5.

Cells were treated with NaF (5 mM) with/without AA or MG149 for 6 h. Cytochrome-c (14 kDa) in the cytosol and in the mitochondria were detected by western blot. (A) AA (30 μM and 50 μM) attenuated fluoride-induced cytochrome-c release in the cytosol. (B) MG149 (30 μM and 50 μM) inhibited fluoride-induced cytochrome-c release in the cytosol. β-actin (44 kDa) was used as a cytosol loading control. VDAC/Porin (35 kDa) was used as a mitochondrial loading control. The numbers show relative protein expression normalized by the loading control (β-actin or VDAC/Porin). Statistical analyses of relative protein expression are shown in Supplementary Fig. 6.