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. 2021 Jan 28;30:0963689720979162. doi: 10.1177/0963689720979162

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Effect of Shikonin on cell apoptosis. (A) Apoptosis and necrosis of QBC939 were measured by flow cytometry with Annexin V/propidium iodide assay. (B) Morphological changes visualized under the fluorescence microscope with Hoechst 33342 staining (magnification 400×). (C) Changes of caspase-3 and caspase-8 mRNA expression were analyzed by real-time polymerase chain reaction (*P < 0.05, compared with the control). (D, E) Expression of pro-caspase-3, activated-caspase-3, and caspase-8 analyzed by Western Blot. (*P < 0.05, **P < 0.01, compared with the control).