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. Author manuscript; available in PMC: 2021 Feb 5.
Published in final edited form as: Cell Rep. 2019 Mar 12;26(11):3145–3159.e5. doi: 10.1016/j.celrep.2019.02.040

Figure 1. Schematic of the gSNA Platform Applied to the Mouse Brain.

Figure 1.

(A) Pipeline and components of genetic single neuron anatomy (gSNA).

(B) Left: scheme of genetic and viral strategy for the labeling of axo-axonic cells (AACs). A transient CreER activity in MGE progenitors is converted to a constitutive Flp activity in mature AACs. Flp-dependent AAVs injected in specific cortical areas enables sparse and robust AAC labeling.

(C) fMOST high-resolution whole-brain imaging. Two-color imaging for the acquisition of GFP (green) channel and PI (propidium iodide, red) channel signals. PI stains brain cytoarchitecture in real time, and therefore provides each dataset with a self-registered atlas. A 488-nm wavelength laser was used for the excitation of both GFP and PI signals. Whole-brain coronal image stacks were obtained by sectioning (with a diamond knife) and imaging cycles at 1-μm z steps, guided by a motorized precision XYZ stage.