Figure 4. Characteristics of L2 AAC Dendrites.

(A) A representative L2 AAC in mPFC. 100-μm max intensity projection. Scale bar: 100 μm.

(B) Dendrites of L2 AAC. Image was enlarged from the box in (A). Scale bar: 50 μm.

(C and D) Apical (C) and main dendrites (D) were enlarged from boxes in (B). Scale bars: 30 μm (C) and 5 μm (D).

(E) Spines (arrows) on the apical dendrites were enlarged from the box in (C). Scale bar: 5 μm.

(F) Complete reconstruction of dendrites (red) and spines (black). The same cell shown in (A) and (B). Inset: enlarged from the box. Black circle: cell body. Gray lines: pia and L1/2 border. Scale bar: 50 μm.

(G) Horizontal dendritic arbor distributions of up-layer (L2 and L3) and deep-layer (L4, L5 and L6) AACs in mPFC, MC, and SSC. Data are mean ± SD.

(H) An example of heatmaps showing the density distribution patterns of a L2 AAC dendritic arbor length (left), branching nodes (middle left), terminal nodes (middle right), and spines (right). Scale bar: 200 μm.

(I) Single-cell density plots of L2 AAC dendrites (same as in H) along the cortical depth.

(J) Density plots of dendrites from 11 L2 AACs in mPFC. Different colors indicate different cells.

(K) Normalized density plots of (J) based on pia and L1/2 border positions (see more details in STAR Methods).

Black circles in (I)–(K) indicate AAC soma positions in the coordinate. Dashed lines correspond to the place of pia (top) and L1/2 border (bottom). Dark black curves in (J) and (K) are averages of all the cells. Density value was presented by ratio.