Upregulation of NUFIP1 mRNA expression (A) and degradation of NUFIP1 protein (B) was observed in tumor bearing mice. NUFIP1 associated factor ZNHIT3 mRNA expression (C) showed no change. Autophagic flux, monitored via changes in LC3-II/I ratio, was higher in ES-2 tumor bearing mice and was driven by a significant reduction in LC3-I (D). Values are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. control.