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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: FASEB J. 2021 Feb;35(2):e21335. doi: 10.1096/fj.202002257R

Fig. 6. NUFIP1 protein, rRNA, and rDNA transcription are reduced in nutrient deprived myotubes.

Fig. 6.

Myotubes exposed for 48 and 72 hrs to 50% ES‐2 unconditioned medium (UCM) or conditioned medium (CM) showed a progressive reduction in rRNA and rDNA transcription (A&B). Nutrient deprivation induced a significant reduction in rRNA within 6 hrs. (C). This reduction in rRNA content was associated with suppressed rDNA transcription, which preceded the reduction in rRNA (~1 hr.) (D). Degradation of NUFIP1 protein was observed within 1 hr. and remained stable by 6 hrs. of nutrient deprivation (E). Autophagic flux was rapidly induced (1 hr.) but equilibrated by 6 hrs. (F). Values are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. DM, # P < 0.05, ## P < 0.01 vs. PBS 1 hr.