Short-term nutrient deprivation in the presence of CLQ rescued degradation of NUFIP1 protein (A) and inhibited autophagic flux (B). However, loss of rRNA was not prevented despite the inhibition of NUFIP1 degradation and autophagic flux, neither was prevented by blocking proteasome activity with MG-132 (Fig C). Values are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs. DM.