Figure 1:

Microfluidic device for local stimulation of lymph-node slices. a) Schematic of key structural features of the lymph node. b) Exploded view of 3-layer PDMS device. Layer 1 contained ten 100 μm x 100 μm microchannels, each with a Y-inlet (Layer 1). Ports in Layer 2 were aligned over the channels, forming the bottom of a perfusion chamber for slice culture (Layer 3). c) Side-view schematic of the device in use. The tissue slice (brown) was perfused with media (blue) above. Stimulus solution (green) was delivered through a microchannel, partially exiting through a port to stimulate a local region of the slice. d) Photograph of assembled device and e) micrograph of the ports aligned over the microchannels. The M-shape design facilitated multiple access points across the lymph node.