Table 1.
Purification summary of bacteriocin produced by Bacillus subtilis (MK733983)
| Purification steps | Volume taken (ml) | Total protein (mg) | Total activity (Au) | Specific activity | Recovery (%) | Purification fold (%) | ZOI (mm) |
|---|---|---|---|---|---|---|---|
| CFS | 100 | 12,800 | 20,500 | 1.6 | 100 | 1 | 15.1 ± 0.2 |
| OPTN | 100 | 15,000 | 25,000 | 1.66 | 121.90 | 1.03 | 20 ± 0.4(33%) ↑ |
| C18 SEP-PACK | 80 | 900 | 8500 | 9.4 | 41 | 5.88 | 22 ± 0.4(47%) ↑ |
| RP-HPLC | 15 | 100 | 7050 | 70.5 | 34.39 | 44.06 | 28.1 ± 0.4(87%) ↑ |
Values are expressed as mean ± SEM (n = 3); Specific activity is the ratio of activity and protein content
ZOI zone of Inhibition; IMO M. smegmatis