Table 3.
Effect of organic solvents, surfactants, preservatives, and metal ions
| Chemical agents | ZOI (mm) | Relative % |
|---|---|---|
| Control | 28.4 ± 0.8 | 100 |
| Blank | 0 | 0 |
| DMSO | 28.2 ± 0.2 | 99.3 |
| Methanol | 28 ± 0.9 | 98.9 |
| Butanol | 28 ± 0.1 | 97.8 |
| Acetone | 27.8 ± 0.2 | 93.3 |
| Benzene | 26.5 ± 0.5 | 97.8 |
| Propanol | 27.8 ± 0.7 | 98.2 |
| Xylol | 27.8 ± 0.2 | 97.8 |
| Chloroform | 27.6 ± 0.5 | 97.1 |
| EDTA | 27.4 ± 0.5 | 96.4 |
| SDS | 16.25 ± 0.5 | 57.21 |
| Triton X 100 | 27.8 ± 0.7 | 97.8 |
| Tween 20 | 23.7 ± 0.2 | 98.38 |
| Tween 80 | 23.7 ± 0.2 | 75.7 |
| sulfobetaine 14 | 27.1 ± 1.04 | 95.42 |
| Sodium deoxycholate | 27.6 ± 0.7 | 97 |
| Sodium citrate | 26.3 ± 0.5 | 92.6 |
| Sodium tungstate | 26.3 ± 1 | 92.5 |
| KCl | 24.1 ± 0.76 | 84.85 |
| CaCl2 | 21.6 ± 1.5 | 75 |
| FeSO4 | 32.5 ± 0.5 | 114.4 |
| ZnSO4 | 18.4 ± 0.5 | 64.7 |
| CuCl2 | 0 | 0 |
| MgSO4 | 15.6 ± 0.5 | 54.9 |
| MnSO4 | 30 ± 1 | 105.6 |
Values are expressed as mean ± SEM (n = 3)
ZOI zone of inhibition; Untreated samples are taken as a positive control–control and LB broth was taken as negative control-Blank; IMO M. smegmatis