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. 2020 Oct 1;15(5):051006. doi: 10.1116/6.0000439

FIG. 3.

FIG. 3.

Changes in the protein corona in response to DNA in solution. (a). Concentration of protein present on the surface of amine-modified NPs (200 nm) following sequential corona formation, with the BSA corona formed first (BSA/DNA), and simultaneous DNA and protein corona (DNA + BSA) formation. BSA, DNA + BSA, and DNA values are replotted from Fig. 2(a). NP concentrations are identical for all experiments and the DNA concentration in solution was 1 mg/ml. (b) Concentration of the protein present on the surface of BSA-NPs (200 nm) following incubation with increasing concentrations of DNA present in the solution. The value for 1 mg/ml DNA is replotted from (a). (c) Concentration of the protein present in the supernatant (Wash 1–3) after addition of DNA to the BSA-NPs for the formation of BSA/DNA-NPs shown in (a). (d) Gel electrophoresis shows the DNA adsorbed on the NP surface for the NPs shown in (a). Equal concentrations of NPs (amine-modified, 200 nm) were loaded for all experiments. Experiments in (a)–(c) were carried out in triplicate and error bars show standard deviation. **p < 0.01, ***p < 0.001, and ****p < 0.0001.