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. 2021 Feb 3;220(4):e202006081. doi: 10.1083/jcb.202006081

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© 2021 Leithner et al.

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Figure S4. — The dependency of T cell priming defects on the pERM-ICAM1-LFA-1 axis are specific to hem1^−/− DCs. (A) Quantification of the number of T cells contacted by mature WT and hem1^−/− DCs. Bars represent the median. Mann-Whitney test, three biological replicates. (B) Flow cytometry histogram for ICAM1 in mature WT and hem1^−/− DCs. (C) Percentages of WT or 1 µM MycB–treated mature DCs activating β2-integrin–deficient T cells, as assessed by CD62L/CD69 surface expression at indicated OVA_323-339 peptide concentrations. (D) Normalized relative number of WT and hem1^−/− DCs in the popliteal lymph node 24 h after coinjection in a 1:2 ratio. t test. Data are pooled from nine different mice. *, P ≤ 0.05; **, P ≤ 0.01; ****, P ≤ 0.0001.