Figure S1.

Intact BBB and microvascular pattern in Lrp1^lox/lox; Tie2-Cre mice at P8, intact microvascular density in 2-mo-old Lrp1^lox/lox; Tie2-Cre mice, and BBB breakdown in 1-mo-old Lrp1^lox/lox; Tie2-Cre mice. (A) Confocal images of lectin⁺ endothelial microvascular profiles in the cortex (green) and retina (red) in 8-d-old (P8) Lrp1^lox/lox; Tie2-Cre and Lrp1^lox/lox littermate control pups injected intravenously with Alexa Fluor 555–conjugated cadaverine show no BBB leakage of tracer into the cortex (red) or retina (green). Representative images shown are from three mice per group. Scale bars = 200 µm. (B and C) Microvascular length (B) and capillary branching points (C) determined in 10-µm-thick cortical tissue sections from P8 Lrp1^lox/lox and Lrp1^lox/lox; Tie2-Cre mice and expressed per square millimeter of tissue. (D) Microvascular length in the cortex of 2-mo-old Lrp1^lox/lox and Lrp1^lox/lox; Tie2-Cre mice and expressed per square millimeter of tissue. (E and F) Quantification of fibrinogen deposits (E) and leakage of Alexa Fluor 555–conjugated cadaverine tracer (F) deposits in the cortex of 1-mo-old Lrp1^lox/lox; Tie2-Cre mice compared with Lrp1^lox/lox controls. In B–F, data represent mean ± SEM, n = 3–5 mice/group; ns, nonsignificant by Student’s t test; **, P < 0.01; ***, P < 0.001.