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. 2021 Jan 22;14:612430. doi: 10.3389/fnbeh.2020.612430

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Copyright © 2021 McMullen, Weiler, Becker and Schirmeier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CG4797 (pippin) and Mfs3 encode putative BBB carbohydrate transporters. (A) RNAi interference-mediated silencing of pippin (using CG4797^dsRNA10598) and Mfs3 Mfs3^dsRNA4726R-3 in selected glial subtypes using glial subtype-specific Gal4 driver lines (pan glial: repo-Gal4; astrocyte-like glial cells (AG): alrm-Gal4; cortex glia (CG), ensheathing glia (EG), and wrapping glia (WG): nrv2-Gal4; subperineurial glial cells (SPG): Gli-Gal4, moody-Gal4; perineurial glial cells (PG): apt-Gal4, 46F-Gal4). Lethality was observed upon panglial and perineurial glia-specific suppression of pippin and Mfs3. In addition, lethality also occurred upon subperineurial glia-specific silencing of pippin. (B) Immunofluorescence staining of a transgenic Pippin-HA adult brain [green: Pippin-HA, magenta: NC120 (PGs and SPGs), blue: laminin (neural lamella)]. Pippin is found in subperineurial and perineurial glial cells. (C) Immunofluorescence staining of an MFS3-EYFP expressing adult brain [green: MFS3-EYFP, magenta: NC120 (PGs and SPGs), blue: laminin (neural lamella)]. MFS3-EYFP is expressed in the perineurial glial cells.