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. 2021 Jan 22;14:612430. doi: 10.3389/fnbeh.2020.612430

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Copyright © 2021 McMullen, Weiler, Becker and Schirmeier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A,B) Pippin and MFS3 facilitate carbohydrate transport. Heterologous expression of Pippin-HA and MFS3-HA in Xenopus oocytes (A) Pippin-HA (B) MFS3-HA are detectable on the surface of Xenopus oocytes (anti-HA immunostaining). (C) Native oocytes show no detectable HA staining. (D,E) Substrate specific uptake of ¹⁴C₁₂-trehalose, ¹⁴C₆-glucose and ¹⁴C₆-fructose into Xenopus oocytes expressing (D) Pippin-HA or (E) MFS3-HA. Both Pippin and MFS3 show considerable transport capacity for trehalose and glucose, but not for fructose. Shown is the net-flux (flux observed in transporter-expressing oocytes minus flux observed in native oocytes). Values represent means ± standard error, N = 1–4.