Figure 3.

pippin and Mfs3 null mutants are viable, but display shortened lifespan and reduced locomotor activity. (A) Survival curves of null mutants and control animals. All mutants show a reduced lifespan compared to control animals, however double pippin^−/−, Mfs3^−/− mutants live significantly longer than single mutants. N = 3, n ≥ 180 (pippin^−/−: p < 10⁻²¹, Mfs3^−/−: p < 10⁻²³, pippin^−/−, Mfs3^−/−: p < 10⁻⁴; log-rank test). (B,C) Activity monitored over 24 h of null mutants and control animals at 2 weeks old (B) and 5 weeks (C) of age. All mutants show a reduction in activity compared to control animals at 2 weeks of age, however the double mutants show a progressive reduction in activity moving significantly less than the single mutant at 5 weeks of age. N = 3; n ≥ 58; **p ≤ 0.01, ***p ≤ 0.001. (D,E) Negative geotaxis assay measuring climbing ability as an escape response of 2 (D) and 5 (E) weeks old flies. Single pippin^−/− or Mfs3^−/− mutants show no reduction in climbing ability at 2 weeks but show a decrease in activity at 5 weeks of age. N = 5; n ≥ 500; ****p ≤ 0.0001.