. 2020 Oct 13;295(50):17251–17264. doi: 10.1074/jbc.RA120.013903

Table 3.

Kinetic parameters for correct and incorrect nucleotide incorporation onto the D-1 DNA substrate catalyzed by hPolε exo− at 20 °C

dNTP	k_max	$K_{d}^{d N T P}$	k_max/ $K_{d}^{d N T P}$	F_pol^a	F_{pol, p261N}/F_pol^b
	s⁻¹	μm	μm⁻¹ s⁻¹
dTTP	411 ± 26	11 ± 2	37	-	-
dATP	0.26 ± 0.01	(4.6 ± 0.5) × 10²	5.7 × 10⁻⁴	1.5 × 10⁻⁵	8
dCTP	-	-	1.4 × 10⁻³	3.8 × 10⁻⁵	8.4
dGTP	(1.50 ± 0.06) × 10⁻²	(1.8 ± 0.1) × 10³	8.3 × 10⁻⁶	2.2 × 10⁻⁷	20

^a Calculated as (k_max/ $K_{d}^{d N T P}$ )_incorrect/[(k_max/ $K_{d}^{d N T P}$ )_correct + (k_max/ $K_{d}^{d N T P}$ )_incorrect].

^b Values for F_{pol, p261N} reported in Table 2 of Ref. 21.