Table 4.

Kinetic parameters for mismatch extension and excision catalyzed by hPolε exo− and hPolε exo+ at 20 °C

dNTP	kmax	${\mathit{K}}_{\mathit{d}}^{\mathit{d}\mathit{N}\mathit{T}\mathit{P}}$	kmax/${\mathit{K}}_{\mathit{d}}^{\mathit{d}\mathit{N}\mathit{T}\mathit{P}}$	Fexta	${\mathit{k}}_{\mathit{o}\mathit{b}\mathit{s}}^{\mathit{d}\mathit{C}\mathit{T}\mathit{P}}$ b	kX	Fexoc
	s−1	μm	μm−1 s−1		s−1	s−1
C:A mismatch (M-1)
dCTP	4.1 ± 0.3	(2.4 ± 0.5) × 102	1.7 × 10−2	-	1.2	-
dGTP	(2.1 ± 0.2) × 10−4	(1.3 ± 0.2) × 103	1.6 × 10−7	9.4 × 10−6	1.5 × 10−5	-
-	-	-	-	-	-	0.4 ± 0.1	0.3
C:C mismatch (M-8)
dCTP	(6.5 ± 0.5) × 10−3	(7 ± 1) × 102	9.3 × 10−6	-	8.1 × 10−4	-
-	-	-	-	-	-	0.5 ± 0.1	617

^a Calculated as (k_max/$K_d^{dNTP}$)_incorrect/[(k_max/$K_d^{dNTP}$)_correct + (k_max/$K_d^{dNTP}$)_incorrect].

^b Calculated as k_max[dNTP]/($K_{\text{d}}^{\text{dCTP}}$ + [dNTP]) during extension from a mismatched primer terminus at an intracellular dCTP concentration of 100 μm.

^c Calculated as k_X/$k_{obs}^{dCTP}$.