Figure 4.

DR1 of LukE binds to DARC. A, hemolysis of primary human erythrocytes treated with 15 nm LukED and increasing concentrations of purified recombinant DARC (n = 4 donors). B, SPR data of the indicated toxins and mutants binding to DARC. LukE^S-DR1 indicates LukE with the DR1 from LukS-PV, LukS^E-DR1 indicates LukS-PV with the DR1 from LukE, etc. The data in A are pooled from two independent experiments and represent means ± S.E. ****, p < 0.0001; ***, p < 0.001. A, one-way ANOVA with Tukey's correction, compared with 0 nm DARC.