Figure 5.

Increased DNA damage in CARM1/PRMT6 double knockout primary MEFs. A, two individual MEF lines generated from WT, CARM1 KO, PRMT6 KO, and DKO embryos were immunostained with anti-γ-H2AX antibody. In a certain field of view, cells with more than five γ-H2AX foci were counted. Also, the average number of γ-H2AX foci per nucleus was calculated and plotted. B, acid-extracted core histones as well as total cellular lysates from WT, CARM1 KO, PRMT6 KO, and DKO MEFs were separated on an SDS-polyacrylamide gel and subsequently immunoblotted with the indicated antibodies.