Figure 1.

Enzymatic characterization of WT FUT8 with a library of glycan acceptors. The upper panel shows a graphical representation of of k_cat/K_m values for enzyme activity using WT FUT8 from Table S1 and the respective glycan acceptor structures shown in the lower panel. The nomenclature abbreviation is shown below each structure and the cartoon representations employ standard symbol nomenclature for glycans (116). Asterisk associated with the M5N2-Asn* structures indicates that this substrate contains an Fmoc modification (see “Experimental Procedures”).