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. 2020 Oct 5;295(50):17128–17137. doi: 10.1074/jbc.RA120.014713

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© 2020 Racca et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 7. — In vitro motility of regulated filament actin on myosin. M8R is shown with filled circles and dotted lines. WT is shown with open circles and solid lines. A and B, velocity of top 5% of filaments (A) and percentage stuck of all filaments (B), each shown with four-parameter Hill fit equations. Each curve represents the average results of three separate experiments. Both cooperativity (n_H: WT 2.64 ± 0.02 versus M8R 1.02 ± 0.01) and calcium sensitivity decreased (pCa₅₀: WT 6.91 ± 0.01 versus M8R 6.62 ± 0.01).