Table 1.

ATP- and calcium-containing solutions for the in vitro motility assay

Component	pCa 4.0	pCa 10.0
BES	25 mm	25 mm
EGTA	5 mm	5 mm
MgCl2	4 mm	4 mm
KOH	40 mm	31.6 mm
KCl	142.8 mm	158 mm
CaCl2	5.095 mm	0 mm
ATP	1 mm	1 mm
DTT	1 mm	1 mm
Glucose	2 mm	2 mm
Glucose oxidase	160 units	160 units
Catalase	2 μm	2 μm
Methyl cellulose	0.5%	0.5%
pH 7.4	High ionic strength: (∼0.21)