Figure 5.
Pre-steady-state kinetic activity of Vpx (−) 2G7, 2N0 RT, and 1M6 RT variants compared with that of WT SIVmac239 RT. Pre-steady-state kinetic values were determined for WT SIVmac239 RT and Vpx (−) RTs 2G7, 2N0, and 1M6 with dTTP using a DNA template (Table 4) as described under “Experimental procedures.” The assays were conducted in triplicate before kpol (A), Kd (B), and incorporation efficiency (kpol/Kd) (C) values of Vpx (−) RTs were compared with those of WT RT. Statistical significance from unpaired two-tailed student's t tests is indicated as: ns, not significant (p < 0.1234); * p < 0.0332; ** p < 0.0021; *** p < 0.002; and **** p < 0.0001.