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Pre-steady-state dTTP incorporation by WT SIVmac239 RT and Vpx (−) RT variants 2G7, 2N0, and 1M6 from DNA template
| RT | Kd (μm) | Kpol (s−1) | Kpol/Kd (s−1 μm−1) |
|---|---|---|---|
| WT | 33 ± 2 | 20.3 ± 1.1 | 0.61 ± 0.01 |
| 2G7 | 16 ± 5 | 35.3 ± 0.4 | 2.3 ± 0.6 |
| 2N0 | 17 ± 3 | 21 ± 3 | 1.3 ± 0.4 |
| 1M6 | 22 ± 4 | 31.5 ± 1.8 | 1.4 ± 0.3 |
