Figure 13.
Effect of silencing endogenous SLC19A2 and SLC19A3 on pyridoxine uptake in Caco-2 cells. Caco-2 cells were cultured for 5 days after transfection with the siRNA for SLC19A2 or SLC19A3. The levels of SLC19A2 mRNA (A) and SLC19A3 mRNA (B) were assessed by real-time PCR. The uptake of [3H]pyridoxine (5 nm) was evaluated for 3 min at pH 5.5 and 37 °C (C). The uptake rate for control was 28.9 fmol/min/mg of protein. Data are presented as the mean ± S.D. (n = 4). Statistical differences from control were assessed by ANOVA followed by Dunnett's test. *, p < 0.05; †, p < 0.1.