Table 1.
Parameters for the inhibition of SLC19A2- and SLC19A3-mediated pyridoxine transport by selected compounds in stable transfectant MDCKII cells
The values of the Hill coefficient (n) and IC50 were determined for the inhibition profiles shown in Fig. 8 and those of the inhibition constant (Ki) were determined for those shown in Fig. 9. The parameter values are presented as the mean ± S.E. (n = 3).
| Compound | Transporter | n | IC50 | Ki |
|---|---|---|---|---|
| Thiamine | SLC19A2 | 0.84 ± 0.07 | 0.71 ± 0.08 µm | 1.11 ± 0.12 µm |
| SLC19A3 | 1.20 ± 0.12 | 0.99 ± 0.14 µm | 0.86 ± 0.12 µm | |
| Pyridoxamine | SLC19A2 | 0.96 ± 0.01 | 55.16 ± 5.64 µm | 90.08 ± 7.77 µm |
| SLC19A3 | 0.94 ± 0.12 | 2.97 ± 0.85 µm | 1.57 ± 0.32 µm | |
| Pyridoxal | SLC19A2 | 0.97 ± 0.03 | 1.49 ± 0.22 mm | 2.63 ± 0.50 mm |
| SLC19A3 | 0.94 ± 0.05 | 0.52 ± 0.04 mm | 0.38 ± 0.03 mm |