Figure 5.

Neither MARK4^wt nor MARK4^ΔG316E317D inhibit the TOR pathway or increase autophagic activity. A, MARK4^ΔG316E317D did not increase the levels of luciferase coexpressed in fly eyes. Western blots were performed on fly heads expressing luciferase alone (luciferase), coexpressing luciferase and MARK4^wt (luciferase+MARK4^wt), or coexpressing luciferase and MARK4^ΔG316E317D (luciferase+MARK4^ΔG316E317D). Actin was used as a loading control. Representative blots and quantitation are shown. Means ± S.D. (error bars); n = 4. n.s. (not significant), p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.005 (one-way ANOVA and Tukey's post-hoc test). B–D, expression of MARK4^wt or MARK4^ΔG316E317D did not promote autophagy. Western blots were performed on fly heads expressing control, MARK4^wt, or MARK4^ΔG316E317D. Blots were performed with anti-pS6K (pS6K) (B), anti-Ref2P (Ref2P) (C), and LC3 antibody (LC3) (D). Representative blots and quantitation are shown. Means ± S.D. (error bars); n = 3. n.s. (not significant), p > 0.05 (one-way ANOVA and Tukey post-hoc test). Actin was used as a loading control.