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Simultaneous deletion of ORC2 and ORC5 in HCT116 p53−/− cells. A, Western blotting of ORC2 and ORC5 in the ORC2+ORC5Δ clones. B, DNA sequences of ORC2 exon 4 show biallelic frameshift mutation of ORC2 in the ORC2+ORC5Δ clones. C, immunoblot of soluble and chromatin-associated proteins in the ORC2+ORC5Δ clones.
