Table 1.
Examples of evolved Taq DNA polymerase variants
Residues in boldface/italic type are found in two or more of the reported Taq DNA polymerase variants. The second column indicates whether variants were generated for specific substrates, such as C2′ modified nucleic acid, or for a specific property, such as thermostability.
| Taq variant | Substrate/Property | Amino acid substitutions | Reference |
|---|---|---|---|
| SFM4-3 | C2′ modification | I614E/E615G/N583S/D655N/E681K/E742Q/M747R | 70 |
| AA40 | RNA, DNA | E602V/A608V/I614M/E615G | 73 |
| M1 | 3′ mismatches | G84A/D144G/K314R/E520G/F598L/A608V/E742G | 74 |
| M4 | 3′ mismatches | D58G, R74P, A109T, L245R, R343G, G370D, E520G, N583S, E694K, A743P | 74 |
| 5D4 | Hydrophobic bases | V62I, Y78H, T88S, P114Q, P264S, E303V, G389V, E424G, E432G, E602G, A608V, I614M, M761T, M775T | 75 |
| H15 | Thermostability | K225E/E388V/K540R/D578G/N583S/M747R | 76 |
| RT-KTQ | m6-A sequencing | L459M/S515R/I638F/M747K | 69 |
| ZP | Z:P substrates | M444V/P527A/E551E/E832V | 65 |