Table 4.
Evaluation of candidate PAK1 genetic interactions with respect to drug resistance using siRNAs
| Serial no. | Gene name | siRNA no. | LDH assaya |
MTT assayb |
||
|---|---|---|---|---|---|---|
| GL26-PAK1-1 | GL26-PAK1-2 | GL26-PAK1-1 | GL26-PAK1-2 | |||
| 1 | ACSL4 | 5 | − | − | − | − |
| 2 | DPP4 | 1 | + | + | + | ++ |
| 3 | ELAVL1 | 5 | − | − | − | − |
| 4 | H2AFX | 1 | − | − | − | − |
| 5 | H3F3A | 1 | − | − | − | − |
| 6 | HSPA8 | 1 | − | − | − | − |
| 7 | JUNB | 2 | − | − | − | − |
| 8 | KIF11 | 5 | − | − | − | − |
| 9 | MCTS1 | 1 | − | − | − | − |
| 10 | METAP2 | 1 | − | − | − | − |
| 11 | mTOR | 1 | + | ++ | + | + |
| 12 | NPC1 | 1 | − | − | − | − |
| 13 | NSUN2 | 3 | − | − | − | − |
| 14 | PKM1 | 4 | − | − | − | − |
| 15 | PKM2 | 6 | − | − | − | − |
| 16 | PLSCR1 | 4 | − | − | − | − |
| 17 | SGPP1 | 1 | + | ++ | + | ++ |
| 18 | TRIP10 | 5 | − | − | − | − |
| 19 | TTK | 1 | ++ | + | ++ | + |
| 20 | YWHAE | 5 | ++ | + | + | + |
a Genetic interaction with p value tested by LDH assay in two independent clones of PAK1 stable transfectants (GL26-PAK1-1 and GL26-PAK1-2) (+, p < 0.05; ++, p < 0.01; −, not significant).
b Genetic interaction with p value tested by MTT assay in two independent clones of PAK1 stable transfectants (GL26-PAK1-1 and GL26-PAK1-2) (+, p < 0.05; ++, p < 0.01; −, not significant).