Figure 2.

Leptin regulates NMDAR activity via Src family kinases. A, representative cell-attached current-clamp recordings from INS-1 832/13 cells treated with leptin (10 nm) alone (top left) or leptin with the PKC inhibitor Go 6983 (bottom left; Go, 10 μm), the CDK5 inhibitor roscovitine (top right; Ros, 10 μm), or the SFK inhibitor AZD (bottom right; 10 μm). Inhibitors were applied for 10 min prior to the application of leptin and remained in the solution throughout the recording. B, group data showing extent of membrane hyperpolarization for leptin alone (n = 40 cells), and leptin co-applied with roscovitine (Ros, n = 14), Go 6983 (n = 18), AZD (n = 17), or dasatinib (Das, 25 μm; n = 15). ***, p < 0.0005 by unpaired t test as compared with leptin. C, representative whole-cell current-clamp recordings of INS-1 832/13 cells without (top trace) or with (bottom trace) the Src kinase–activating peptide YEEI (1 μm) in the pipette solution. D, group data showing the degree of hyperpolarization for control (n = 6) and YEEI peptide (n = 7). *, p < 0.05, unpaired t test as compared with control.