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Parameters governing fidelity for T7 DNA polymerase: WT versus E514Cou
| Enzyme | Nucleotide | kpol (s−1) | Kd,app (µm) | kpol/Kd,app (m−1 s−1) | Fidelity |
|---|---|---|---|---|---|
| WT | Correct (dATP:T) | 181 (154, 221) | 28.1 (20.4, 41.0) | 6.4 × 106 (3.9, 10.8) | 0.61 × 10−6 (1.03, 0.35) |
| Mismatch (dATP:A) | -- | 3.90 (3.78, 4.02) | |||
| E514Cou | Correct (dATP:T) | 202 (188, 225) | 15.0 (12.8, 18.2) | 13.5 × 106 (10.3, 17.6) | 1.56 × 10−6 (2.10, 1.56) |
| Mismatch (dATP:A) | -- | 21.0 (20.3, 21.7) | |||
Parameter (best-fit (lower boundary, upper boundary)).
