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The structure of OTUB1 and E2. (A) Crystal structure of OTUB1. (B) Crystal structure of OTUB1-ubiquitin aldehyde-UBC13~Ub. (C) Superposition of OTUB1 structures in (A,B). Ubiquitin (yellow), UBC13 (green), OTUB1 without ubiquitin (white-blue), OTUB1 with ubiquitin (blue), N-terminal ubiquitin-binding helix (blue) is disordered in the apoenzyme. (PDB: 4DHZ and 2ZFY).
