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. 2021 Jan 20;22(3):1004. doi: 10.3390/ijms22031004

Figure 4.

Figure 4

Canstatin inhibited the OGD/R-induced activation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) in NRCMs. NRCMs were stimulated with OGD/R (see the protocols described in Figure 3). Control cells were cultured in the reperfusion medium under normoxic conditions (Cont; (A,B)). PBS (Cont, OGD/R; (A,B)), canstatin (+canstatin: 250 ng/mL; (A)), gp91 ds-tat (+gp91 ds-tat: 2.5 µM; (B)) or tempol (+tempol: 1 mM; (B)) was treated throughout the OGD/R stimulation. Total cell lysates of the NRCMs were harvested and NOX activity was determined by lucigenin assay. The normalized NOX activity relative to PBS was shown as mean ± S.E.M. ((A): n = 6, (B): n = 4). *, ** p < 0.05, 0.01 vs. Cont, #, ## p < 0.05, 0.01 vs. OGD/R.