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. 2020 Dec 11;27(2):gaaa074. doi: 10.1093/molehr/gaaa074

Figure 3.

Figure 3.

let-7d is downregulated in endometria of intrauterine adhesions (IUA) patients and prevents epithelial–mesenchymal transition (EMT) in vitro. (A) Relative expression of let-7d in endometria of normal controls (n = 18) and severe IUA patients (n = 18). (B) Relative expression of let-7d in TGF-β-treated Ishikawa (IK) cells at the indicated point of time (n = 3). (C) Relative mRNA expression of E-cad (n = 4), (D) α-SMA (n = 4), (E) FN (n = 4) and (F) Left: western blot analysis of E-cad, N-cad, α-SMA and FN proteins levels in TGF-β-pretreated IK cells transfected with let-7d mimic (TGF-β + let-7d) or microRNA negative control (TGF-β + miR-NC) for 48 h. Right: Quantification of corresponding proteins (n = 3) determined by image J software. The data are shown as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Student’s t-tests were performed for panels A, C to F. For panel B, one-way ANOVA was performed. The number above the asterisk in each panel is the fold difference.