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. 2021 Jan 20;22(3):1015. doi: 10.3390/ijms22031015

Figure 3.

Figure 3

Influence of AChEI and memantine on enteric neurons. (a) Neurons were treated on DIV3 for 24 h with the indicated drug or DMSO as a solvent control (ctrl). Viability was analyzed by the CellTiter Glo assay (n = 4 donor C57B6L/J wild type mice, aged 2–3 months). (b) Neurite mass was assessed after 24 h growth with the respective drug. Cells were cultivated on transwell plates and neurite mass determined after stripping off cell bodies by crystal violet stain (n = 4 donor mice). (c) Calcium influx into enteric neurons treated for 25 min with the respective drugs or DMSO was measured by calcium-stain after KCl injection into the medium. Slope of the fluorescent signal in % of the solvent control is shown. (n = 8–11 per group, 4 donor mice). Data are presented as mean + SEM. Statistical analysis was conducted by One Way ANOVA with Dunnett’s multiple comparison test (* p < 0.05).