Figure 1.

Multiple domains in PLCε are required for Rap1A^G12V-dependent activation.A, domain diagram of rat PLCε. The Y-box insertion and C2-RA1 linker are shown in orange and purple, respectively. Numbers above the diagram correspond to the domain boundaries most relevant to this work, with the variants under study shown below. B, PLCε PH-COOH (black circles) is activated by Rap1A^G12V in a concentration-dependent manner. In contrast, PH-C2 (red squares) and EF3-COOH (blue triangles) are not activated at any concentration of Rap1A^G12V tested. The data represents at least two independent experiments performed in duplicate. Error bars represent S.D.